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computer-assisted zeiss microscope  (Carl Zeiss)
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Carl Zeiss computer-assisted zeiss microscope
Karyotypes of two invasive rat mammary carcinomas and of four neoplastic clones evolved from non-clonal hyperplasias in vitro. The karyotypes of the two rat mammary carcinomas RMT 47–3 (A) and 37–2 (B) were prepared from short-term cultures of the two carcinomas treated with colcemid for several hours following published procedures [ , ]. Prior to karyotyping the metaphase chromosomes were hybridized with DNA probes carrying chromosome-specific fluorescent colors . The chromosomes were then arranged into conventional karyotypes with a computer-assisted Zeiss <t>microscope</t> [ , ]. The karyotypes of the four clonal neoplasias RMT-CN 65 (C), RMT-CN 58 (D) , RMT-CN 54 (E) and RMT-CN 61 (F), which had evolved spontaneously from cultures of preneoplastic hyperplasias propagated in vitro, were prepared as described for the two carcinomas.
Computer Assisted Zeiss Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Karyotypes of two invasive rat mammary carcinomas and of four neoplastic clones evolved from non-clonal hyperplasias in vitro. The karyotypes of the two rat mammary carcinomas RMT 47–3 (A) and 37–2 (B) were prepared from short-term cultures of the two carcinomas treated with colcemid for several hours following published procedures [ , ]. Prior to karyotyping the metaphase chromosomes were hybridized with DNA probes carrying chromosome-specific fluorescent colors . The chromosomes were then arranged into conventional karyotypes with a computer-assisted Zeiss microscope [ , ]. The karyotypes of the four clonal neoplasias RMT-CN 65 (C), RMT-CN 58 (D) , RMT-CN 54 (E) and RMT-CN 61 (F), which had evolved spontaneously from cultures of preneoplastic hyperplasias propagated in vitro, were prepared as described for the two carcinomas.

Journal: Molecular Cytogenetics

Article Title: Karyotypic evolutions of cancer species in rats during the long latent periods after injection of nitrosourea

doi: 10.1186/s13039-014-0071-x

Figure Lengend Snippet: Karyotypes of two invasive rat mammary carcinomas and of four neoplastic clones evolved from non-clonal hyperplasias in vitro. The karyotypes of the two rat mammary carcinomas RMT 47–3 (A) and 37–2 (B) were prepared from short-term cultures of the two carcinomas treated with colcemid for several hours following published procedures [ , ]. Prior to karyotyping the metaphase chromosomes were hybridized with DNA probes carrying chromosome-specific fluorescent colors . The chromosomes were then arranged into conventional karyotypes with a computer-assisted Zeiss microscope [ , ]. The karyotypes of the four clonal neoplasias RMT-CN 65 (C), RMT-CN 58 (D) , RMT-CN 54 (E) and RMT-CN 61 (F), which had evolved spontaneously from cultures of preneoplastic hyperplasias propagated in vitro, were prepared as described for the two carcinomas.

Article Snippet: The chromosomes were then arranged into conventional karyotypes with a computer-assisted Zeiss microscope [ , ].

Techniques: Clone Assay, In Vitro, Microscopy

Cellular morphologies of two rat mammary carcinomas and of four neoplastic clones evolved from rat hyperplasias in vitro. Cells of the rat mammary carcinomas RMT 47–3 and 37–2 and of four clonal neoplasias derived from mammary hyperlasias termed RMT-CN 65, RMT-CN 58, RMT-CN 54 and RMT-CN 61 were photographed in cell culture dishes at a magnification of 100x with a phase-contrast microscope. The following individualities were observed: (A) A dense monolayer of polygonal cells of the RMT 47–3 carcinoma, (B) Three-dimensional colonies of round and refractile cells of the carcinoma RMT 37–2 clone 1, (C) A dense monolayer of fusiform cells of the clonal neoplasia RMT-CN 65, (D) A three-dimensional multilayer of pleomorphic-round cells of the clonal neoplasia RMT-CN 58, (E) A relatively flat monolayer of fusiform, triangular cells of the clonal neoplasia RMT-CN 54, (F) A three-dimensional cell layer of elongated refractile cells of the clonal neoplasia RMT-CN 61.

Journal: Molecular Cytogenetics

Article Title: Karyotypic evolutions of cancer species in rats during the long latent periods after injection of nitrosourea

doi: 10.1186/s13039-014-0071-x

Figure Lengend Snippet: Cellular morphologies of two rat mammary carcinomas and of four neoplastic clones evolved from rat hyperplasias in vitro. Cells of the rat mammary carcinomas RMT 47–3 and 37–2 and of four clonal neoplasias derived from mammary hyperlasias termed RMT-CN 65, RMT-CN 58, RMT-CN 54 and RMT-CN 61 were photographed in cell culture dishes at a magnification of 100x with a phase-contrast microscope. The following individualities were observed: (A) A dense monolayer of polygonal cells of the RMT 47–3 carcinoma, (B) Three-dimensional colonies of round and refractile cells of the carcinoma RMT 37–2 clone 1, (C) A dense monolayer of fusiform cells of the clonal neoplasia RMT-CN 65, (D) A three-dimensional multilayer of pleomorphic-round cells of the clonal neoplasia RMT-CN 58, (E) A relatively flat monolayer of fusiform, triangular cells of the clonal neoplasia RMT-CN 54, (F) A three-dimensional cell layer of elongated refractile cells of the clonal neoplasia RMT-CN 61.

Article Snippet: The chromosomes were then arranged into conventional karyotypes with a computer-assisted Zeiss microscope [ , ].

Techniques: Clone Assay, In Vitro, Derivative Assay, Cell Culture, Microscopy